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"On- Array and In-solution methods for DNA capture for focused re-sequencing of targeted genomic regions using Next Generation Sequencing Technologies"
- by
Dr. Henner Friedle, (Genomic Applications Scientist EMEA), Agilent Technologies
Abstract: Massively parallel DNA sequencing technologies are of pivotal importance in genome biology and medicine, as they can potentially enable comprehensive and systematic evaluation of genetic variation. Currently, these sequencing technologies are geared toward sequencing whole genomes. A broader adoption of these technologies requires a more cost-effective method with higher throughput and greater versatility than PCR—a method such as target enrichment, the targeted resequencing of multiple discrete genomic regions of interest. To address this, Agilent has released the highly powerful SureSelect Target Enrichment System that offers two different approaches:
The in-solution system was developed in collaboration with the Broad Institute. This system uses an extremely efficient hybrid selection technique which significantly improves the cost- and process efficiency of the sequencing workflow, allowing for a larger number of samples per study.
For smaller projects Agilent validated the use of DNA microarrays for target enrichment in collaboration with the laboratory of Dr. Greg Hannon (Cold Spring Harbor Laboratory). We targeted 0.025% of the human genome, using an Agilent 244K array of 60-mer probes to capture approximately 1,287 discrete genomic regions. The captured DNA was then released and sequenced. The results obtained by this study confirm that Agilent DNA microarrays can provide a rapid and effective solution for targeted sequencing of genomic regions of interest.
Date and Time: Friday, 7th August, 2009; 4 P.M to 4.45 P.M
High Tea and Interactive session: 4:45 P.M onwards
Co-ordinator: Dr. Parag P. Sadhale, IISc
Venue: MCB Lecture Hall, IISc
Conducted by: MCB Society, IISc
Sponsored by: Genotypic Technology Pvt Ltd., Bangalore in collaboration with Agilent technologies
RSVP: Ms. Tanuka Bhattacharjee
email:
Telephone: +91 - 80 - 40538300
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