Nanopore Sequencing


About MinION

MinION is the only portable, real time device for DNA and RNA sequencing.

Each consumable flow cell can now generate 5-10 GB of DNA sequence data. Ultra-long read lengths are possible (hundreds of kb) as you can choose your fragment length.  The MinION streams data in real time so that analysis can be performed during the experiment and workflows are fully versatile.

The MinION weighs under 100g and plugs into a PC or laptop using a high speed USB 3.0 cable. No additional computing infrastructure is required.  Not constrained to a laboratory environment, it has been used up a mountain, in a jungle, in the arctic and on the International Space Station.


About Nanopore DNA Sequencing :

Direct molecular analysis 

Nanopore sequencing data starts streaming immediately. Being able to sequence the native DNA/RNA helps to avoid errors introduced by amplification and other preparation procedures. It also has potential uses in the analysis of modified bases.



The MinION can be used outside the traditional lab environment to perform field-based work - taking the analysis to the sample


The MinION can be run for minutes or days according to the experimental need. Users can adjust settings like the speed that the DNA passes through the nanopore.


Long read lengths 

The Oxford Nanopore system processes the fragments that are presented to it rather than generating specific read lengths that are limited by the reading chemistry itself. The longest read reported by a MinION user to date is more than 200Kb, reflecting the length of that DNA fragment, but it can process the user-controlled distribution of fragment lengths generated during sample preparation.

Real-time data  

Nanopore sequencing data starts streaming immediately, rather than being delivered in bulk at the end of a 'run'. The implications of this on workflows and fast turnaround results has been explored in many applications. For example, returning an answer on a species identification very quickly, or allowing sequencing experiments to be stopped if sufficient data has accumulated to answer a question, so you can move on to the next experiment.


Application of Long Reads

1. Microbial whole genome sequencing

  • De novo assembly
  • Scaffolding and finishing
  • Variant analysis: structural variation
  • Resequencing

2. Metagenomics (16s and WGS)
3. Large Amplicons Sequencing
4. Methylation
5. Genome Gap Closure
6. Direct RNA Sequencing
7. Epigenetics

  • Methylation
8. RNA sequencing
  • Splice variant analysis
  • Transcriptome / gene expression
  • Fusion transcript analysis


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